Quantifying Neurofibrillary Tangles via Thioflavin T

Thioflavin T staining method was employed to quantify the levels of neurofibrillary tangles (NFTs). Tissue sections were brought to room temperature and washed three times for 5 min each in tbs (A510025, Sangon Biotech). Then, the tissue sections were immersed in a light-protected solution of 0.0125% Thioflavin T in ethanol (HY-D0972, MedChemExpress) for 8 min. Subsequently, the tissue sections were washed three times for 5 min each in 50% ethanol, followed by one wash in TBS for 5 min. After drying the tissue sections in a light-protected manner, glycerin was used for sealing. The staining results were observed and photographed under a fluorescence microscope (Olympus, Tokyo, Japan).