Gene expression profiles from fresh-frozen biopsy sections of 46 tumours and normoxia- and hypoxia-treated cell lines were derived using Illumina bead arrays HT-12 v4 (Illumina Inc., San Diego, CA, USA) with ∼47 300 transcripts, as described by Halle et al (2012) (link). Total RNA was isolated using the RNeasy MiniKit (Qiagen, Hilden, Germany). RNA quality control was performed with Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). cRNA was synthesised, labelled and hybridised to the arrays. Signal extraction and quantile normalisation were carried out using the software provided by the manufacturer (Illumina Inc.). Log-transformed data were used in all analyses. The data have been deposited to the Gene Expression Omnibus (GEO) repository (GSE55935).
Protocol detail
Hypoxia-Induced Gene Expression Profiling
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Oslo University Hospital
Top 5 similar protocols
Protocol cited in 14 other protocols
Variable analysis
independent variables
- Normoxia- and hypoxia-treated cell lines
- Gene expression profiles from fresh-frozen biopsy sections of 46 tumours and normoxia- and hypoxia-treated cell lines
- Total RNA was isolated using the RNeasy MiniKit (Qiagen, Hilden, Germany)
- RNA quality control was performed with Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA)
- CRNA was synthesised, labelled and hybridised to the arrays
- Signal extraction and quantile normalisation were carried out using the software provided by the manufacturer (Illumina Inc.)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!