Antibody Validation for LDLR and Viral Proteins

Mouse anti-Flag monoclonal antibody (#F3165-M2, Sigma-Aldrich), rabbit anti-β-actin monoclonal antibody (#AC026, ABclonal), rabbit anti-hLDLR monoclonal antibody (#10231-R301, SinoBiological), rabbit anti-hLDLR monoclonal antibody (#A14996, ABclonal), rabbit anti-mLDLR monoclonal antibody (#50305-R004, SinoBiological), mouse anti-IFNAR1 monoclonal antibody (MAR1-5A3, #BE0241, BioXCell), mouse anti-Gn monoclonal antibody (JE12, #MAB12317, The Native Antigen Company), phycoerythrin (PE)-conjugated anti-hLDLR monoclonal antibody (#10231-R301-P, SinoBiological), Alexa Fluor™ 488-conjugated anti-mouse IgG (#R37114, Thermo Fisher), human control IgG (#12000C, Invitrogen), mouse control IgG (#I5381, Sigma-Aldrich), rabbit control IgG (#CR1, SinoBiological) and goat control IgG (#CR2, SinoBiological) were purchased from the indicated companies. Human anti-Gc monoclonal antibody ADI-36121 was prepared as previously reported^{15 (link)} and provided by Dr. Bing Yan at Wuhan Institute of Virology, Chinese Academy of Sciences. Mouse anti-Gn monoclonal antibody (clone 7A11) was customized from ABclonal and further validated by us.

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Xu Z.S., Du W.T., Wang S.Y., Wang M.Y., Yang Y.N., Li Y.H., Li Z.Q., Zhao L.X., Yang Y., Luo W.W, & Wang Y.Y. (2024). LDLR is an entry receptor for Crimean-Congo hemorrhagic fever virus. Cell Research, 34(2), 140-150.

Publication 2024

rabbit anti-β-actin Alexa Fluor™ 488-conjugated anti-mouse IgG mouse control IgG

Corresponding Organization :

Other organizations : Wuhan Institute of Virology, Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Mouse anti-Flag monoclonal antibody
Rabbit anti-hLDLR monoclonal antibody
Rabbit anti-mLDLR monoclonal antibody
Mouse anti-IFNAR1 monoclonal antibody
Mouse anti-Gn monoclonal antibody
PE-conjugated anti-hLDLR monoclonal antibody
Alexa Fluor™ 488-conjugated anti-mouse IgG
Human anti-Gc monoclonal antibody ADI-36121
Mouse anti-Gn monoclonal antibody (clone 7A11)

dependent variables

Not explicitly mentioned

control variables

Rabbit anti-β-actin monoclonal antibody
Human control IgG
Mouse control IgG
Rabbit control IgG
Goat control IgG

positive controls

Human anti-Gc monoclonal antibody ADI-36121

negative controls

Human control IgG
Mouse control IgG
Rabbit control IgG
Goat control IgG

Annotations

Based on most similar protocols

Use appropriate isotype controls for antibodies to account for non-specific binding (Protocol 1, 2, 3, 4, 5).

Validate antibodies by Western blot or flow cytometry to confirm specificity and sensitivity (Protocol 1, 2, 3, 4, 5).

Use endotoxin-free reagents and glassware to minimize non-specific immune activation (Protocol 1).

Optimize antibody concentrations for flow cytometry and microscopy applications (Protocol 1, 2, 4).

Utilize purified or recombinant proteins as positive controls to validate target-specific responses (Protocol 4, 5).

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