Total RNA Extraction and Transcriptome Sequencing of N. yunnanensis

Total RNA was extracted from young leaves of the same individual N. yunnanensis tree using a CTAB-pBIOZOL method [17 (link)]. The purity, concentration, and integrity of RNA samples were measured on a NanoDrop One UV-Vis spectrophotometer (Thermo Fisher Scientific, USA), a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, USA) and with Agilent 2100 Bioanalyzer on-chip electrophoresis (Agilent Technologies, Inc; Santa Clara, CA) [18 (link)], respectively, to ensure that the samples qualified for transcriptome sequencing. The cDNA library was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA), and then sequenced on a DNBSEQ-G50 platform at BGI-Shenzhen (BGI Co. Ltd., Shenzhen, China), resulting in ∼8.85 Gb of raw transcriptome data (150 bp, paired-end). All of the raw sequence reads were further filtered using SOAPfilter v2.2 (SOAP, RRID:SCR_000689) with the parameters “-y -q 33 -i 200 -g 1 -M 2 -Q 20” to remove the adapters and low-quality reads.