Pericytes Protect GBM Cells from TMZ

Pericytes (5 × 10⁴) and GBM cells (5 × 10⁴) were, respectively, cultured in the upper or lower chamber in 6-well transwell apparatus, followed by the treatment of TMZ (500 μmol/L, Selleckchem, S1237). To determine the protective effect of pericytes or CCL5 on GBM cells, GBM cells were pretreated with or without pericyte CM, recombinant human CCL5 (10 ng/mL, R&D), or MVC (500 nmol/L, Selleckchem, S2003), followed by TMZ (500 μmol/L) treatment 4 h later. Apoptotic analysis was performed using Annexin-V Apoptosis Detection Kit (Invitrogen, 88-8007-74) according to the manufacturer’s instructions. Cell survival analysis was performed using CCK-8 (Dojindo, CK04) as previously described.^{48 (link)}