Protein Expression Analysis by Western Blot and IHC

Western blotting and IHC analysis were performed as described previously (Cao et al., 2018 (link)). The antibodies used were as follows: anti-FNDC3A antibody (Abcam, Cambridge, United Kingdom), anti-VEGFA antibody (Proteintech, Wuhan, China), anti-OPN3 antibody (Affinity Biosciences, Cincinnati, United States), and anti-CPE antibody (Proteintech, Wuhan, China). The IHC quantization analysis was calculated by ImageJ software and statistically analyzed in three random fields. Data are shown as mean ± SEM.

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Yu J., Liang L.L., Liu J., Liu T.T., Li J., Xiu L., Zeng J., Wang T.T., Wang D., Liang L.J., Xie D.W., Chen D.X., An J.S, & Wu L.Y. (2021). Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer. Frontiers in Genetics, 12, 733715.

Publication 2021

anti-VEGFA antibody

Anti antibody Antibodies

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

Other organizations : Beijing Red Cross Blood Center

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Variable analysis

independent variables

Anti-FNDC3A antibody
Anti-VEGFA antibody
Anti-OPN3 antibody
Anti-CPE antibody

dependent variables

Western blotting analysis
IHC analysis

control variables

Experiments were performed as described previously (Cao et al., 2018)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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