B. thetaiotaomicron was routinely cultured under
anaerobic conditions at 37 °C using an anaerobic cabinet (Whitley A35
Workstation; Don Whitley, UK) in culture volumes of 0.2, 2 or 5 ml) of TYG
(tryptone-yeast extract-glucose medium) or minimal medium containing 1% of
an appropriate carbon source plus 1.2 mg/ml porcine hematin (Sigma-Aldrich)
as previously described5 (link). The growth
of the cultures were routinely monitored at OD600nm using a
Biochrom WPA cell density meter (Cambridge, UK) for the 5 ml cultures or a
Gen5 v2.0 Microplate Reader (Biotek) for the 0.2 and 2 ml cultures.
anaerobic conditions at 37 °C using an anaerobic cabinet (Whitley A35
Workstation; Don Whitley, UK) in culture volumes of 0.2, 2 or 5 ml) of TYG
(tryptone-yeast extract-glucose medium) or minimal medium containing 1% of
an appropriate carbon source plus 1.2 mg/ml porcine hematin (Sigma-Aldrich)
as previously described5 (link). The growth
of the cultures were routinely monitored at OD600nm using a
Biochrom WPA cell density meter (Cambridge, UK) for the 5 ml cultures or a
Gen5 v2.0 Microplate Reader (Biotek) for the 0.2 and 2 ml cultures.
