Plastome Sequencing of Diverse Cacti

To avoid resource waste caused by repeated sampling, we accessed the European Nucleotide Archive (ENA) (https://www.ncbi.nlm.nih.gov/sra) database and obtained WGS (whole-genome sequencing) data for 9 species with a complete plastome assembly. They all come from the Kew Tree of Life project [61 (link)], which is freely available for use. Furthermore, we collected fresh stem samples of 26 cactuses from flower markets in Chongqing, Guangxi, and Fujian in September 2020. All specimens were deposited in the Herbarium of Southwest University, Chongqing. Two previously reported species (Carnegiea gigantea, NC_027618.1; Lophocereus schottii, NC_041727.1) were also included in our analysis. The details of the plant samples used for plastome assembly are given in Table S5.

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Yu J., Li J., Zuo Y., Qin Q., Zeng S., Rennenberg H, & Deng H. (2023). Plastome variations reveal the distinct evolutionary scenarios of plastomes in the subfamily Cereoideae (Cactaceae). BMC Plant Biology, 23, 132.

Publication 2023

Cactuses European Nucleotide Plant Stem Tree

Corresponding Organization : Southwest University

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Variable analysis

independent variables

Whole-genome sequencing (WGS) data for 9 species with a complete plastome assembly
Fresh stem samples of 26 cactuses collected from flower markets in Chongqing, Guangxi, and Fujian in September 2020

dependent variables

Plastome assembly and analysis

control variables

Two previously reported species (Carnegiea gigantea, NC_027618.1; Lophocereus schottii, NC_041727.1) included in the analysis

controls

Positive control: Two previously reported species with known plastome assemblies
Negative control: Not explicitly mentioned

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