The following antibody and lectins were utilized in this study: Rabbit anti-cone arrestin (cones; Millipore; AB15282; 1:1000: RRID:AB_1163387), mouse (IgG1) anti-calsenilin (type 4 cone bipolar cells; Millipore; 05–756; 1:500: RRID:AB_309969), mouse (IgG1) anti-Goa (ON bipolar cells; Millipore; MAB3073; 1:1000: RRID:AB_94671), Peanut agglutinin (PNA) (cone pedicles; Molecular Probes; L21409; 1:1000: RRID:AB_2315178), rabbit anti-PkaRIIβ (type 3b bipolar cells; BD biosciences 610625; 1:500: RRID:AB_397957), rabbit anti-PKCα (rod bipolar cells; Santa Cruz Biotechnology; sc-10800; 1:500: RRID:AB_2168560). DAPI was included in the second post-secondary antibody wash at a dilution of 1:50,000 of a 1 mg/ml stock (RRID:AB_2307445). Secondary Antibodies: Secondary antibodies were acquired from Jackson Immuno-Research (RRID:SCR_010488) and used at a concentration of 1:1000. The specificity of antibody reagents used in this study have previously been experimentally verified and described in detail (de Andrade et al., 2014 (link)).
Protocol detail
Retinal Neurotransmitter Receptor Localization
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Antibodies
Antibody
Antibody specificity
Arrestin
Calsenilin
Cells
Cone
Dapi
Dilution
Igg1
Lectins
Molecular probes
Mouse
Peanut agglutinin
Pkcα
Rabbit
Rod cells
Corresponding Organization : University of Washington
Other organizations : University of Louisville
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Variable analysis
independent variables
- Rabbit anti-cone arrestin
- Mouse (IgG1) anti-calsenilin
- Mouse (IgG1) anti-Goa
- Peanut agglutinin (PNA)
- Rabbit anti-PkaRIIβ
- Rabbit anti-PKCα
- Localization and expression of cone arrestin
- Localization and expression of calsenilin
- Localization and expression of Goa
- Localization and expression of cone pedicles
- Localization and expression of PkaRIIβ
- Localization and expression of PKCα
- DAPI staining
- The specificity of antibody reagents used in this study have previously been experimentally verified and described in detail (de Andrade et al., 2014).
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