Multiple alignments of the conserved RCC1 protein sequences of bread wheat, emmer wheat, and Aegilops tauschii were performed using Clustal Omega (Sievers et al., 2011 (link)
) using default parameters, and a phylogenetic tree was constructed using a maximum-likelihood method with 1,000 bootstrap replications in the RaxML_NG software (Kozlov et al., 2019 (link)
). Figtree 1.4.4 (http://tree.bio.ed.ac.uk/software/figtree/) was used to visualize and optimize the phylogenetic tree.
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