Plasmid Constructs for Cyclin D1 and G9a

The pBIND plasmids expressing GAL4-tagged cyclin D1 wild type and mutations were as described previously (57 (link)). The pcDNA3.1 plasmids encoding FLAG-tagged G9a^WT and deletion mutations were obtained from Dr. Eiji Hara (Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan) (58 (link)). The pLKO lentiviral plasmid vectors of human G9a shRNA and GLP shRNA were purchased from Sigma.

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Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.

Publication 2019

pLKO lentiviral plasmid vectors of human G9a shRNA and GLP shRNA

Cancer Cyclin d1 Deletion mutations Human Japanese Mutations Plasmid Shrna Vectors

Corresponding Organization : Nanyang Technological University

Other organizations : Baruch S. Blumberg Institute, Thomas Jefferson University, Drexel University, Cincinnati Children's Hospital Medical Center, University of Stuttgart

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Variable analysis

independent variables

GAL4-tagged cyclin D1 wild type and mutations
FLAG-tagged G9a^WT and deletion mutations

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None specified
Negative control: None specified

Annotations

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