Spindle angles were determined using FIJI by finding the distance between the mitotic spindle poles: (i) in the xy axis of the flattened ventrolateral ectoderm in a sum projection of the confocal z stack; (ii) in z axis by determining the z planes that the 2 poles appear. The mitotic spindle orientation angle, φ, was calculated by taking the inverse tan of the difference in z depth divided by the distance in xy between the spindle poles (Fig EV3C and D ).
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Mitotic Spindle Angle Measurement
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Other organizations : University of Rochester, University of Rochester Medical Center
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Variable analysis
independent variables
- Mitotic spindle orientation angle, φ
- Distance between the mitotic spindle poles in the xy axis of the flattened ventrolateral ectoderm in a sum projection of the confocal z stack
- Difference in z depth between the 2 spindle poles
- Not explicitly mentioned
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