Determining Optimal Bacteriophage Infection MOI

To determine the optimal MOI, the bacterial culture was infected with the bacteriophages at 5 different MOI ratios: 0.001, 0.01, 0.1, 1 and 10. The infected cells were incubated for 6 h at 37 °C with mild agitation (170 rpm) after incubation; the suspension was centrifuged for 10 min at 10,000 rpm using Beckman Coulter^® Microfuge^® 16 Centrifuge to obtain clear supernatants for the bacteriophage titer assay. The MOI with the highest bacteriophage titer was taken as the optimal MOI. All the experiments were performed in triplicate and the results were taken as the average of the three replicates [22 (link)].