Protocol detail

Murine CTCL Xenograft Model Development

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We developed a murine xenograft model of CTCL using immune-deficient mice (NOD.CB17-Prkdc^SCID). The CD4⁺ Myla cell line was generated from a patient with mycosis fungoides and purchased from MilliporeSigma (catalog 95051032). For controls, we purchased another 2 lymphoma cell lines from ATCC: Hut78 (ATCC, catalog CRM-TIB-161, generated from a patient with Sézary syndrome) and Hut 102 (ATCC, catalog TIB-162.1, generated from a patient with MF lymphoma). In CB17 mice, CTCL was generated via an intradermal injection of CD4⁺ Myla cells (1 × 10⁵ cells/μL, 100 μL) into the nape of the neck (44 (link)). Tumor growth, measured by tumor volume, was assessed for 60 days.

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Chen O., He Q., Han Q., Furutani K., Gu Y., Olexa M, & Ji R.R. (2023). Mechanisms and treatments of neuropathic itch in a mouse model of lymphoma. The Journal of Clinical Investigation, 133(4), e160807.

Publication 2023

Hut78 Hut 102

Cd4 cells Cell lines Cells Ctcl Intradermal injection Lymphoma Mice Mycosis fungoides Neck Patient Sézary syndrome Tumor Xenograft

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Variable analysis

independent variables

CD4+ Myla cell line injection into mice

dependent variables

Tumor growth (measured by tumor volume)

control variables

Immune-deficient NOD.CB17-Prkdc^SCID mice
Hut78 cell line (from patient with Sézary syndrome)
Hut 102 cell line (from patient with MF lymphoma)

controls

Positive control: Hut78 cell line (from patient with Sézary syndrome)
Positive control: Hut 102 cell line (from patient with MF lymphoma)
Negative control: Not explicitly mentioned

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