The ECO formulation was prepared by dispersing 1.2% w/w Carbopol C934P and 2.4% w/w Pluronic® F127 in water under vigorous stirring until complete homogenization occurred. Then, the pH was adjusted to 7 using TEA. Afterward, COR was slowly added until it reached 20% w/w, and the system was continuously stirred for 30 min (Scheme 1). After manufacturing, the emulgel (called ECO, a gel containing COR) was stored at room temperature. The details of the optimization and preparation have recently been published [12 (link)].
Campanholi K.D., Junior R.C., de Morais F.A., Gonçalves R.S., Rodrigues B.M., Pozza M.S., de Castro-Hoshino L.V., Leite S.M., Capeloto O.A., Baesso M.L., Pozza P.C, & Caetano W. (2023). Copaiba Oil-Based Emulsion as a Natural Chemotherapeutic Agent for the Treatment of Bovine Mastitis: In Vivo Studies. Pharmaceutics, 15(2), 346.
Corresponding Organization : Universidade Estadual de Maringá
Other organizations :
Centre de Recherches sur les Macromolécules Végétales, Université Grenoble Alpes, Centre National de la Recherche Scientifique, Universidade Federal do Maranhão, Universidade Federal do Amazonas
No positive or negative controls were explicitly mentioned.
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