The F. graminearum strain W3008 was kindly provided by the College of Plant Science and Technology of Huazhong Agricultural University, China [59 (link)]. The strain was routinely cultured at 25 °C on potato dextrose agar (PDA, Hopebio, Qingdao, China) plates and was preserved in 20% disinfected glycerol at −80 °C for long-term storage [60 (link)]. Thymol (HPLC grade standard, purity > 98%, B21153, Shanghai yuanye Bio-Technology Co., Ltd. Shanghai, China) was dissolved in acetone into a 100 mg/mL stock solution, protected from light, and stored at 4 °C.
Free full text: Click here