TGF-β1 and SIRT1 Levels in Kidney Tissue

TGF-β1 and SIRT1 levels of kidney homogenized tissue were measured by ELISA method (Zellbio, Germany). In this case, the measurement was based on the reaction between the antigen and the antibody, and finally the adsorption was read at 450 nm. After placing the adsorption and concentration of standard solutions in Excel program, the standard curve was prepared. Concentration of the samples was determined based on the adsorption using the standard curve linear Eqs. [40 (link)–42 (link)].

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Sabet N., Soltani Z., Khaksari M, & Raji-Amirhasani A. (2022). The effects of two different dietary regimens during exercise on outcome of experimental acute kidney injury. Journal of Inflammation (London, England), 19, 2.

Publication 2022

ELISA method

Adsorption Antibody Antigen Elisa Kidney Sirt1 Tgf β1 Tissue

Corresponding Organization :

Other organizations : Kerman University of Medical Sciences

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Variable analysis

independent variables

TGF-β1 levels
SIRT1 levels

dependent variables

TGF-β1 levels
SIRT1 levels

control variables

Adsorption of standard solutions at 450 nm

controls

Positive control: Standard solutions
Negative control: Not specified

Annotations

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