A skin biopsy was performed on the patient who reported cutaneous involvement. The fibroblast cells were isolated from skin biopsies and kept in liquid nitrogen until used as previously described.15 (link) Briefly, fibroblasts were cultivated in Dulbecco's Modified Eagle Medium with 10% fetal bovin serum (Seradigm) with 100 IU/mL penicillin G (Sigma-Aldrich) and 25 μg/mL gentamicin (Gemini Bio-Products) in 8% CO2 at 37°C. They were cultured in flasks until confluency (100%) prior to be split and seeded in a 6-well plate containing sterile glass coverslips.