The immortalized normal human cholangiocyte cell line, H69, was a gift from Dr. G.J. Gores, Mayo Clinic, Rochester, MN. Immortalized murine normal pooled cholangiocyte lines were also used.(10 (link)) Human LSCs were purchased from Creative Bioarray (Shirley, NY). The human hepatic stellate cells,(20 (link)) human mesenchymal stem cells (MSCs) and human hepatocytes (HHs) were purchased from Sciencell (Carlsbad, CA). The human acute monocytic leukemia cell line (THP-1) was purchased from ATCC Inc. (Manassas, VA) and maintained in Roswell Park Memorial Institute 1640 medium (Sigma-Aldrich) with 10% fetal calf serum (FCS) without antibiotics.
Protocol detail
Immortalized Cell Lines for Liver Research
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Acute monocytic leukemia
Antibiotics
Cell line
Fetal calf serum
Hepatic stellate cells
Hepatocytes
Human
Mesenchymal stem cells
Murine
Corresponding Organization : Texas A&M University
Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, Humanitas University
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Variable analysis
independent variables
- Immortalized normal human cholangiocyte cell line, H69
- Immortalized murine normal pooled cholangiocyte lines
- Not explicitly mentioned
- Human LSCs
- Human hepatic stellate cells
- Human mesenchymal stem cells (MSCs)
- Human hepatocytes (HHs)
- Human acute monocytic leukemia cell line (THP-1)
- Positive control: None mentioned
- Negative control: None mentioned
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