Comprehensive Western Blot Analysis

Western blot was performed as described previously [24 (link)].The primary antibodies were purchased from Cell signaling including β-catenin(#8480S), snai2(#9585S), vimentin (#5741S), surviving (#2808S), XIAP(#14334S), cIAP1(#7065S), cIAP2(#3130S), livin (#5471S), cleaved-PARP(#5625S), cleaved-caspase3(#9661S), T-SMAD2/3 (#8685S), P-SMAD2 (#18338S). GAPDH was purchased from Sigma (#G9545) and cytokeratin-7 was purchased from Abcam (#ab181598).

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Zhao G., Wang Q., Wu Z., Tian X., Yan H., Wang B., Dong P., Watari H., Pfeffer L.M., Guo Y., Li W, & Yue J. (2019). Ovarian primary and metastatic tumors suppressed by survivin knockout or a novel survivin inhibitor. Molecular cancer therapeutics, 18(12), 2233-2245.

Publication 2019

β-catenin snai2 vimentin surviving cIAP1 cIAP2 livin cleaved-PARP cleaved-caspase3 T-SMAD2/3 P-SMAD2 GAPDH cytokeratin-7

Antibodies Caspase3 Ciap1 Ciap2 Cytokeratin 7 Gapdh Livin Smad2 Vimentin Western blot β catenin

Corresponding Organization : Henan University

Other organizations : Iowa State University, Third Affiliated Hospital of Zhengzhou University, Hokkaido University

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Variable analysis

independent variables

Primary antibodies purchased from Cell Signaling including β-catenin(#8480S), snai2(#9585S), vimentin (#5741S), surviving (#2808S), XIAP(#14334S), cIAP1(#7065S), cIAP2(#3130S), livin (#5471S), cleaved-PARP(#5625S), cleaved-caspase3(#9661S), T-SMAD2/3 (#8685S), P-SMAD2 (#18338S)

dependent variables

Protein expression levels of the target proteins identified by the primary antibodies

control variables

GAPDH (purchased from Sigma #G9545) as a loading control
Cytokeratin-7 (purchased from Abcam #ab181598) as a control protein

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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