Rapamycin (LC Laboratories) was reconstituted in absolute ethanol at 10 mg/mL and stored at −20°C. Rapamycin was diluted in 5.2% Tween 80 (Sigma) and 5.2% polyethylene glycol (PEG-400; Hampton Research) and injected i.p. (1 mg/kg) every other day for 15 days [20 (link), 68 (link)]. Only vehicle solution was administered as controls.
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