Protein of cancer cells or tissues was prepared using 1× cell lysis buffer (Promega). Western blotting was performed as previously reported [12 (link), 13 (link), 49 (link)–51 (link)], with antibodies specific for NONO (ab70335), ERG (ab92513), Ets-1 (ab26096), FLAG (ab45766), HA (ab9110, Abcam Inc., Cambridge, MA), histone H3 (sc-10809), GST (sc-33614), and β-actin (sc-130300, Santa Cruz Biotechnology, Santa Cruz, CA).
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