Cells were treated with recombinant human IL-1β (R&D Systems, Abingdon, U.K.) and recombinant rat IFNγ (R&D Systems, Abingdon, U.K.) for 24 hr. The concentrations of cytokines (Marroqui et al., 2014 (link)) used are indicated in the figures.
The synthetic dsRNA analog PIC (Invitrogen, San Diego, CA, USA) was used at the final concentration of 1 μg/ml and its transfection into cells was performed under the same conditions as used for siRNA (see below) but using 0.15 ml of Lipofectamine 2000 (Colli et al., 2011 (link)).
Culture supernatants were collected for nitrite determination (nitrite is a stable product of nitric oxide [NO] oxidation) at OD540 nm using the Griess method (Green et al., 1982 (link)).
The synthetic dsRNA analog PIC (Invitrogen, San Diego, CA, USA) was used at the final concentration of 1 μg/ml and its transfection into cells was performed under the same conditions as used for siRNA (see below) but using 0.15 ml of Lipofectamine 2000 (Colli et al., 2011 (link)).
Culture supernatants were collected for nitrite determination (nitrite is a stable product of nitric oxide [NO] oxidation) at OD540 nm using the Griess method (Green et al., 1982 (link)).
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