Reactive oxygen species (ROS) detection was performed using the ROS detection kit (Beyotime Institute of Biotechnology). Cell suspensions were collected and stained with the ROS probe 2,7-dichlorofluorescein diacetate (DCFDA; Beyotime Institute of Biotechnology) as previously described.21 (link) Following 20 min of incubation, cells were centrifuged at 300 x g, washed with PBS and resuspended. Finally, the relative ROS levels were measured by flow cytometry (BD Biosciences), and the data were analyzed using FlowJo 10.0 software (FlowJo LLC).
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Measurement of Intracellular ROS Levels
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Corresponding Organization : First Affiliated Hospital of Chinese PLA General Hospital
Other organizations : First Affiliated Hospital of Harbin Medical University
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Variable analysis
independent variables
- ROS detection kit (Beyotime Institute of Biotechnology)
- Relative ROS levels
- Cell suspensions
- ROS probe 2,7-dichlorofluorescein diacetate (DCFDA; Beyotime Institute of Biotechnology)
- Incubation time (20 min)
- Centrifugation (300 x g)
- PBS washing
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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