Ex vivo experiments were performed on six squirrel monkeys. The brains were perfusion fixed with 4% paraformaldehyde preceded by rinse with physiological saline. Brains were then removed and immersed for 3 weeks in phosphate-buffered saline (PBS) medium with 1mM Gd-DTPA in order to reduce longitudinal relaxation times (D’Arceuil et al. 2007 (link)). The brains were then placed in liquid Fomblin (California Vacuum Technology) and scanned on the same Varian 9.4 T magnet. Ex vivo imaging has several experimental advantages including longer scanning times and absence of motion. Together, this allows acquisition of data with higher signal-to-noise ratios and at a higher resolution compared to in vivo studies. We chose to include ex vivo templates because of their significantly increased contrast, and the additional value of the use of ex vivo and subsequent histology as a means to validate MRI techniques and aid image interpretation (Azadbakht et al. 2015 (link); Bastiani et al. 2016 (link); Calabrese et al. 2015 (link); Choe et al. 2012 (link); Knosche et al. 2015 (link); Leergaard et al. 2010 (link); McNab et al. 2009 (link); Schilling et al. 2016 (link)).