Oligodendrocyte Viability Assay with GW4064

Primary OPCs were isolated using the immunopanning method as described earlier [22 (link), 23 ]. OPCs/differentiating oligodendrocytes were incubated with 1 or 10 μM GW4064 (Tocris, 2473) for 24/48 h. Cell viability upon treatment was measured according to manufacturer’s protocol using CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7570). When treated with bone marrow-derived macrophage (BMM) supernatant, oligodendrocytes were incubated with 50% supernatant in culture medium for 48 h.

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Albrecht S., Fleck A.K., Kirchberg I., Hucke S., Liebmann M., Klotz L, & Kuhlmann T. (2017). Activation of FXR pathway does not alter glial cell function. Journal of Neuroinflammation, 14, 66.

Publication 2017

GW4064 CellTiter-Glo® Luminescent Cell Viability Assay

Bone marrow derived macrophage Cell viability Culture medium Gw4064 Luminescent assay Oligodendrocytes Promega

Corresponding Organization :

Other organizations : University Hospital Münster, University of Münster

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Variable analysis

independent variables

GW4064 concentration (1 μM or 10 μM)
Treatment duration (24 h or 48 h)

dependent variables

Cell viability

control variables

Cell type (OPCs/differentiating oligodendrocytes)
Culture medium

controls

Positive control: Not specified
Negative control: Not specified

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