A total of 43 compounds were selected for analysis, 36 of which are classified as HPHCs by the FDA [12 ]. This included the nine compounds on the FDA-CTP’s list of HPHCs relevant for smokeless tobacco products: acetaldehyde, arsenic, B(a)P, cadmium, crotonaldehyde, formaldehyde, nicotine (total and unprotonated), NNK, and NNN [13 ]. We also screened for some other compounds covered by the GOTHIATEK standard (aflatoxin B2, G1 and G2, nitrite, and ochratoxin A) [7 ]. In addition, N-nitrosoanatabine (NAT), and N-nitrosoanabasine (NAB) were included to cover all four, primary TSNAs. All reported results are based on wet weight.
GOTHIATEK also includes maximum levels for a large number of agrochemicals. Analyses of such compounds were considered beyond the scope of the current study.
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