Fasting Biomarker Measurement Protocol

After maintaining a fasting state for 12 hours, blood samples were obtained from each participant to measure plasma glucose, HbA1C, creatinine, and lipid profiles. Serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol (LDL-C) were determined using the dry, multilayer analytical slide method in the Fuji Dri-Chem 3000 analyzer (Fuji Photo Film Corporation, Tokyo, Japan). The levels of HbA1C were evaluated by ion-exchange high-pressure liquid chromatography method (BIO-RAD VARIANT II, Los Angeles, CA). Plasma glucose concentrations were determined by the glucose oxidase method on a Beckman Glucose Analyzer II (Beckman Instruments, Fullerton, CA).

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Liu J.S., Su S.C., Kuo F.C., Li P.F., Huang C.L., Ho L.J., Chen K.C., Liu Y.C., Lin C.P., Cheng A.C., Lee C.H., Lin F.H., Hung Y.J., Liu H.Y., Lu C.H, & Hsieh C.H. (2023). The efficacy and safety of combined GLP-1RA and basal insulin therapy among inadequately controlled T2D with premixed insulin therapy. Medicine, 102(10), e33167.

Publication 2023

Fuji Dri-Chem 3000 analyzer Beckman Glucose Analyzer II

Blood Cholesterol Creatinine Glucose Glucose oxidase High pressure liquid chromatography Ion exchange Lipid Low density lipoprotein cholesterol Plasma Serum Triglyceride

Corresponding Organization : Tri-Service General Hospital

Top 5 similar protocols

Variable analysis

independent variables

Fasting state

dependent variables

Plasma glucose
HbA1C
Creatinine
Serum total cholesterol
Serum triglyceride
Serum low-density lipoprotein cholesterol (LDL-C)

control variables

Fasting duration (12 hours)

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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