DNA Isolation and SNP Genotyping

DNA was isolated from peripheral blood using the QIAmp DNA mini kit (Qiagen GmbH), following the manufacturer's instructions. DNA (10 ng) was mixed with TaqMan Universal PCR Master mix II and TaqMan SNP probes (Applied Biosystems; Thermo Fisher Scientific, Inc.) and genotypes of SNPs rs2749812 were analysed with the 7500 Fast Real-Time Polymerase Chain Reaction (PCR) system with allelic discrimination according to the protocol used in a previous publication (7 (link)).

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Alehagen U., Shamoun L, & Wågsäter D. (2020). Genetic variance and plasma concentration of CD93 is associated with cardiovascular mortality: Results from a 6.7-year follow-up of a healthy community-living elderly population. Molecular Medicine Reports, 22(6), 4629-4636.

Publication 2020

QIAmp DNA mini kit TaqMan Universal PCR Master mix II TaqMan SNP probes

Allelic Blood Discrimination Genotypes Polymerase chain reaction Real time polymerase chain reaction Snps

Corresponding Organization :

Other organizations : Linköping University, Region Jönköpings län, Uppsala University

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Variable analysis

independent variables

SNP rs2749812

dependent variables

Genotypes of SNPs rs2749812

control variables

DNA (10 ng) was mixed with TaqMan Universal PCR Master mix II and TaqMan SNP probes (Applied Biosystems; Thermo Fisher Scientific, Inc.)
Genotypes were analysed with the 7500 Fast Real-Time Polymerase Chain Reaction (PCR) system with allelic discrimination

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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