Protocol detail

Protective mAbs against C. albicans

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We identified and isolated a panel of protective mouse monoclonal antibodies (mAbs) specific for C. albicans cell surface peptides and a glycan epitope, and each mAb is protective in both immunocompetent mice and neutropenic mice. Therefore, we selected the following four conserved 14-mer protective peptide epitopes and one glycan epitope as targets for human IgGs: Fba (YGKDVKDLFDYAQE), Met6 (PRIGGQRELKKITE), Hwp1, and phosphoglycerate kinase 1 (Pgk1) (VPLDGKTITNNQRI). Each synthetic peptide was produced commercially (GenScript) and used as the coating antigen on enzyme immunoassay (EIA) plates. The purity of each peptide was >98.5%. The glycan epitope β-1,2-mannotriose (gift from David Bundle) was conjugated to bovine serum albumin (BSA) as a trimannose-BSA conjugate for EIA plate coating.

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Xin H., Rosario-Colon J.A, & Eberle K. (2023). Novel Intravenous Immunoglobulin Therapy for the Prevention and Treatment of Candida auris and Candida albicans Disseminated Candidiasis. mSphere, 8(1), e00584-22.

Publication 2023

Antigen Bovine serum albumin Cell Enzyme immunoassay Epitope Glycan Human Immunocompetent Mannotriose Mice Peptide Peptides c Phosphoglycerate kinase

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Variable analysis

independent variables

Synthetic peptides (Fba, Met6, Hwp1, Pgk1)
Glycan epitope β-1,2-mannotriose conjugated to bovine serum albumin (BSA)

dependent variables

Protective effects of mouse monoclonal antibodies (mAbs) against C. albicans in immunocompetent and neutropenic mice

control variables

Purity of synthetic peptides (>98.5%)
Coating of EIA plates with synthetic peptides and glycan epitope-BSA conjugate

controls

Positive control: Mouse monoclonal antibodies (mAbs) specific for C. albicans cell surface peptides and glycan epitope
Negative control: Not explicitly mentioned

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