Fabrication of Lipid Bilayers on Chromium Barriers

Chrome barriers were deposited on quartz microscope slides via e-beam lithography and thermal evaporation, as described (63 (link), 84 (link)). Lipid bilayers were prepared with 91.5% 1,2-dioleoyl-sn-glycero-3-phosphocholine, 0.5% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), and 8% 1,2-dioleoyl-sn-glycero-3-phosphoethanoloamine-N-[methoxy(polyethyleneglycol)-2,000] (Avanti Polar Lipids, Inc., catalog nos. 850375P, 870273P, and 880130P, respectively). Lipid bilayers were deposited in preformed flow chambers through sequential deposition of a lipid master mix in lipid buffer (20 mM Tris-Cl [pH 7.5], 100 mM NaCl).

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Meir A., Crickard J.B., Kwon Y., Sung P, & Greene E.C. (2022). Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments. Proceedings of the National Academy of Sciences of the United States of America, 119(4), e2113871119.

Publication 2022

1,2-dioleoyl-sn-glycero-3-phosphocholine 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N

Buffer Dioleoyl phosphoethanolamine Lipid Lipid a Lipid bilayers Microscope Nacl Polyethyleneglycol Quartz Sn glycero 3 phosphocholine Tris

Corresponding Organization : Columbia University

Other organizations : The University of Texas Health Science Center at San Antonio

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Variable analysis

independent variables

Chrome barrier deposition on quartz microscope slides via e-beam lithography and thermal evaporation

dependent variables

Lipid bilayer formation

control variables

Lipid composition: 91.5% 1,2-dioleoyl-sn-glycero-3-phosphocholine, 0.5% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), and 8% 1,2-dioleoyl-sn-glycero-3-phosphoethanoloamine-N-[methoxy(polyethyleneglycol)-2,000]
Lipid buffer composition: 20 mM Tris-Cl (pH 7.5), 100 mM NaCl

controls

No positive or negative controls were explicitly mentioned in the provided information.

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