Integrin Gene Expression Analysis

To explore integrin α2, αV and β1 gene expression, RNA from HepG2 and Huh7 was isolated using RNeasy Mini Kit (Qiagen, Hilden, Germany), according to [11 (link)].
The iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA) and gene specific primers (Integrin α2, αV, β1, GAPDH; Bio-Rad Laboratories) were employed: ITGA2 UniqueAssayID: qHsaCID0016134; ITGAV UniqueAssayID: qHsaCID0006233; ITGB1 UniqueAssayID: qHsaCED0005248; GAPDH UniqueAssayID: qHsaCED0038674. RNA levels were normalized with GAPDH and calculated in relative quantification as ∆∆Ct (elative fold change in gene expression).

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Juratli M.A., Zhou H., Oppermann E., Bechstein W.O., Pascher A., Chun F.K., Juengel E., Rutz J, & Blaheta R.A. (2022). Integrin α2 and β1 Cross-Communication with mTOR/AKT and the CDK-Cyclin Axis in Hepatocellular Carcinoma Cells. Cancers, 14(10), 2430.

Publication 2022

RNeasy Mini Kit iTaq Universal SYBR Green Supermix GAPDH

Gapdh Gene Gene expression Integrin α2 Primers Sybr green

Corresponding Organization : Johannes Gutenberg University Mainz

Other organizations : Goethe University Frankfurt, University Hospital Münster

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Variable analysis

independent variables

Cell lines: HepG2 and Huh7

dependent variables

Gene expression levels of integrin α2, αV and β1

control variables

GAPDH expression used for normalization of gene expression levels

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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