Western blotting was performed as described previously [45 (link)]. The antibodies were mouse anti-β-actin (BOSTER, Wuhan, China), rabbit anti-ID4 (Abcam, Cambridge, MA, USA), rabbit anti-PCNA (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Cyclin D1 (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Caspase-3 (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt/pAkt (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China), and anti-mouse/anti-rabbit secondary antibodies (Fcmacs, Nanjing, China).
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