Investigating p53 Regulation via miR-142-3p

The WT and mutated p53-coding regions were cloned and then inserted into the EcoRI and SpeI sites of the pGL3-luciferase vector, which was kindly provided by Dr Peifeng Li (Institute of Zoology, CAS, Beijing, China). The WT p53-coding region was mutated from ‘CACUGC’ to ‘GUGAUG’. Wild-type or mutated pGL3-p53 CDS co-transfected with negative control or the miR-142-3p duplex (Invitrogen, Shanghai, China) were transfected into HEK293T cells using Lipofectamine2000 (Invitrogen). The luciferase activity was measured using the luciferase activity assay kit (Promega). Luciferase and GFP reporter assay in zebrafish was conducted as described previously [24 (link), 58 (link)].