Versatile AAV Viral Vector Constructs

AAV1-CaMKII-Cre was obtained from the University of Pennsylvania Viral Vector Core (Cat#AV-1-PV2396). AAV1-CAG-FLEX-GFP and AAV1-CAG-FLEX-tdTomato were obtained from Addgene (#51502-AAV1, #51503-AAV1). pAAV-FLEX-GFP (Addgene #28304) and pAAV-FLEX-tdTomato (Addgene #28306) were gifts from Edward Boyden (Massachusetts Institute of Technology). AAVDJ/8-CAG-FLEX-tdTomato and AAVDJ/8-CAG-FLEX-GFP were produced with pAAV2-1 and purified as described previously [38 (link), 39 (link)].

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Hasegawa R., Ebina T., Tanaka Y.R., Kobayashi K, & Matsuzaki M. (2020). Structural dynamics and stability of corticocortical and thalamocortical axon terminals during motor learning. PLoS ONE, 15(6), e0234930.

Publication 2020

AAV1-CAG-FLEX-tdTomato pAAV-FLEX-GFP pAAV-FLEX-tdTomato

Camkii Cat 1 Gifts Tdtomato Vector

Corresponding Organization : RIKEN Center for Brain Science

Top 5 similar protocols

Variable analysis

independent variables

AAV1-CaMKII-Cre
AAV1-CAG-FLEX-GFP
AAV1-CAG-FLEX-tdTomato
AAVDJ/8-CAG-FLEX-tdTomato
AAVDJ/8-CAG-FLEX-GFP

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

PAAV-FLEX-GFP (Addgene #28304) and pAAV-FLEX-tdTomato (Addgene #28306) were gifts from Edward Boyden (Massachusetts Institute of Technology)

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