Hydromethanolic Extraction of Abutilon Leaves

The plant leaves after the collection were dried under shade and crushed to a fine powder. About 1.2 kg powder was macerated in 60% methanol (Sigma Aldrich, USA) for 15 days. After 15 days the plant extract was further filtered via Whatman filter paper. After filtration, the extract was concentrated using a rotary evaporator (B-490, Buchi, Switzerland) at 45 °C and obtained 122 g greenish A. parviflora hydromethanolic (APHM) leaves extract [18 (link), 19 (link)]. The extract was stored at 4 °C for further studies.

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Burki S., Burki Z.G., Asghar M.A., Ali I, & Zafar S. (2021). Phytochemical, acute toxicity and renal protective appraisal of Ajuga parviflora hydromethanolic leaf extract against CCl4 induced renal injury in rats. BMC Complementary Medicine and Therapies, 21, 198.

Publication 2021

methanol B-490

Filtration Methanol Plant Plant extract Powder

Corresponding Organization : Jinnah Sindh Medical University

Other organizations : Federal Urdu University, International Center for Chemical and Biological Sciences, University of Karachi

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Variable analysis

independent variables

Drying of plant leaves under shade
Crushing of dried plant leaves to a fine powder
Maceration of 1.2 kg powder in 60% methanol for 15 days
Filtration of plant extract via Whatman filter paper
Concentration of extract using a rotary evaporator at 45 °C

dependent variables

Yield of greenish Achyranthes parviflora hydromethanolic (APHM) leaves extract (122 g)

control variables

Temperature of rotary evaporation (45 °C)
Storage of extract at 4 °C for further studies

controls

No positive or negative controls were explicitly mentioned in the provided information.

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