Phenolic Profiling of Graciano Extract

The phenolic composition of the Graciano extract (GE) was studied by ultra-high performance liquid chromatography and mass spectrometry using an ACQUITY UPLC^TM system with a diode array detector and coupled to a quadrupole time of flight mass spectrometer (Waters, Milford, MA, USA). The separation was carried out as described by Asensio-Regalado et al. [15 (link)] Briefly, the mobile phases were 0.1% (v/v) acetic acid in MeOH and 0.1% (v/v) acetic acid in water delivered at a flow rate of 0.35 mL/min; the volume injected was 5.0 µL; flavan-3-ols, hydroxycinnamic acids, and flavanols were detected at 280, 320, and 370 nm, respectively. Positive and negative ion modes mass spectra were recorded.