Flow Cytometry Analysis of Cell Markers

Flow cytometry was conducted as previously described [3 (link)]. Cultured cells were stained with 0614 mAb or human chimera 0614-5 mAb or IE9 mAb as the primary antibody and Alexa Fluor 488- or 647-conjugated anti-rat or human or mouse-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody. Stained cells were analyzed on a Guava easyCyte 10HT (Merck Millipore). Dead cells were stained using propidium iodide (PI) (Thermo Fisher Scientific). Data were analyzed using the FlowJo software (Tree Star, Tokyo, Japan).

Free full text: Click here

Anzai T., Saijou S., Takashima H., Hara M., Hanaoka S., Matsumura Y, & Yasunaga M. (2022). Identification of CD73 as the Antigen of an Antigen-Unknown Monoclonal Antibody Established by Exosome Immunization, and Its Antibody–Drug Conjugate Exerts an Antitumor Effect on Glioblastoma Cell Lines. Pharmaceuticals, 15(7), 837.

Publication 2022

Guava easyCyte 10HT propidium iodide (

Alexa fluor 488 Antibody Cells Chimera Cultured cells Flow cytometry Guava Human Mouse Propidium iodide Tree

Corresponding Organization : Chiba Cancer Center

Other organizations : Nippon Seiki (Japan)

Top 5 similar protocols

Variable analysis

independent variables

Primary antibody: 0614 mAb, human chimera 0614-5 mAb, or IE9 mAb

dependent variables

Cell staining profile analyzed by flow cytometry

control variables

Cell culture conditions
Secondary antibody: Alexa Fluor 488- or 647-conjugated anti-rat, human, or mouse-Ig polyclonal antibody
Instrument used for flow cytometry: Guava easyCyte 10HT
Viability stain: Propidium iodide (PI)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!