Luciferase reporter vectors (circNINL-WT, circNINL-MUT, FGFR1 3'UTR-WT and FGFR1 3'UTR-MUT) were generated by inserting circNINL or FGFR1 3'UTR sequence containing miR-3918 target site or mutated site into pGL3 vector (Promega). The vectors were co-transfected into A549 cells with miR-3918 mimic by Lipofectamine® 3000, and after 48 h, cell luciferase activity was checked by dual luciferase detection system kit (Promega) [28 (link)].
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