Western Blotting Protein Detection

Western blotting was performed as described in previous publications ^{76 (link)–78 (link)}. Antibodies used were rabbit anti-human FOXM1 (1:1000, 5436; Cell Signaling Technology), rabbit anti-human cyclin B1 (1:1000, 12231, Cell Signaling Technology), and rabbit anti-human β-actin (1:2000, 4970; Cell Signaling Technology). Resultant immunological bands were observed using the ChemiDoc XRS Plus System (Bio-Rad Laboratories). The intensity of the bands was quantified using ImageJ software (National Institutes of Health, Bethesda, MD). Uncropped, full-length original gel images are shown in Supplementary Figs. S1 and S2.

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Ito T., Tanaka Y., Kaku-Ito Y., Oda Y, & Nakahara T. (2024). FOXM1: a new therapeutic target of extramammary Paget disease. Scientific Reports, 14, 4048.

Publication 2024

rabbit anti-human β-actin ChemiDoc XRS Plus System

Corresponding Organization :

Other organizations : Kyushu University

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Variable analysis

independent variables

Antibodies used: rabbit anti-human FOXM1, rabbit anti-human cyclin B1, and rabbit anti-human β-actin

dependent variables

Intensity of the immunological bands observed using the ChemiDoc XRS Plus System (Bio-Rad Laboratories) and quantified using ImageJ software

control variables

Western blotting procedure, as described in previous publications

controls

Positive control: None specified
Negative control: None specified

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