Autophagy and Aggrephagy Assays in HeLa and Huh7 Cells
Corresponding Organization : Osaka University
Variable analysis
- Bafilomycin A1 (125 nM)
- Puromycin (5 μg/mL, 4 h)
- Torin-1 (250 nM)
- Rutin (dissolved in 42% ethanol)
- Quercetin (dissolved in DMSO and 42% ethanol)
- LC3 flux
- Aggrephagy
- HeLa and Huh7 cell lines
- Dulbecco's modified Eagle's medium (DMEM) with 10% FBS
- Incubation in 5% CO2 at 37 °C
- HeLa cells stably expressing ULK1-EGFP, GFP-Atg5, GFP-WIPI, tf-LC3, and GFP-TFEB
- Not explicitly mentioned
Annotations
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