Cre-Dependent AAV Viruses for Interneuron Targeting

AAV viruses used in this study were as follows: AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (UPenn AV-1–20298P), AAV1.hysn.hChR2(H134R)-eYFP.WPRE.hGH (UPenn AV-26973P), AAV1.EF1a.DIO.eYFP.WPRE.hGH (Upenn AV-1–27056), AAVrg.CAG.GFP (Addgene 37825-AAVrg), AAVrg.CAG.tdTomato (Addgene 59462-AAVrg). Additional viral constructs were assembled for Cre-dependent expression of a reporter under the control of the Dlx5/6 enhancer: AAV-Dlx-Flex-GFP (Addgene #83900) and AAV-Dlx-Flex-ChR2-mCherry (Dimidschstein et al., 2016 (link)). These constructs take advantage of the double-floxed inverted system, in which two consecutive and incompatible lox-sites are placed both in 5' and 3' of the reversed coding sequences of the viral reporter, restricting expression to Cre-expressing interneurons.

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Liu X., Dimidschstein J., Fishell G, & Carter A.G. (2020). Hippocampal inputs engage CCK+ interneurons to mediate endocannabinoid-modulated feed-forward inhibition in the prefrontal cortex. eLife, 9, e55267.

Publication 2020

AAVrg.CAG.GFP AAV-Dlx-Flex-GFP

Coding sequences Hysn Interneurons Tdtomato Viruses

Corresponding Organization :

Other organizations : New York University, Broad Institute, Massachusetts Institute of Technology, Harvard University

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Variable analysis

independent variables

AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH
AAV1.hysn.hChR2(H134R)-eYFP.WPRE.hGH
AAV1.EF1a.DIO.eYFP.WPRE.hGH
AAVrg.CAG.GFP
AAVrg.CAG.tdTomato
AAV-Dlx-Flex-GFP
AAV-Dlx-Flex-ChR2-mCherry

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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