Antibody Detection of Influenza Proteins

Polyclonal antibodies were used to detect TRIM22 (Sigma-Aldrich; HPA003575), Mx1 (Santa Cruz; sc-50509), GBP1 (Proteintech; 15303-1-AP), IFIH1 (Proteintech; 21775-1-AP), TLR3 (Proteintech; 17766-1-AP), and actin (Sigma-Aldrich; A5060). IAV hybridoma antisera were used to detect NP, M1, and NS1, as previously described (Turnbull et al., 2016 (link)). Monoclonal antibodies were used to detect UBA7 (AbCam; ab133499), Actin (DSHB; 224-236-1), IFITM1 (Proteinech; 60074-1g), and IAV NP (AbCam; ab20343). Secondary antibodies were Alexa 488 and 555 donkey anti-mouse and -rabbit (Invitrogen; A21202, A21206, and A31572), DyLight 680- or 800-conjugated anti-rabbit (Thermo Fisher Scientific; 35568 and SA5-35571), and peroxidase conjugated anti-mouse (Sigma-Aldrich; A4416).

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Charman M., McFarlane S., Wojtus J.K., Sloan E., Dewar R., Leeming G., Al-Saadi M., Hunter L., Carroll M.W., Stewart J.P., Digard P., Hutchinson E, & Boutell C. (2021). Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection. Frontiers in Cellular and Infection Microbiology, 11, 689707.

Publication 2021

sc-50509 A5060 ab20343 A21202 A21206 A31572 A4416

Actin Antibodies Antisera Donkey Hybridoma Ifih1 Ifitm1 Monoclonal antibodies Mouse Peroxidase Rabbit

Corresponding Organization : MRC University of Glasgow Centre for Virus Research

Other organizations : Children's Hospital of Philadelphia, University of Pennsylvania, Roslin Institute, University of Edinburgh, University of Liverpool, University of Al-Qadisiyah, Public Health England

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Variable analysis

independent variables

Polyclonal antibodies used to detect TRIM22, Mx1, GBP1, IFIH1, TLR3, and actin
IAV hybridoma antisera used to detect NP, M1, and NS1
Monoclonal antibodies used to detect UBA7, Actin, IFITM1, and IAV NP

dependent variables

Detection of TRIM22, Mx1, GBP1, IFIH1, TLR3, actin, NP, M1, NS1, UBA7, Actin, IFITM1, and IAV NP

control variables

Not explicitly mentioned

positive controls

IAV hybridoma antisera used to detect NP, M1, and NS1

negative controls

Not explicitly mentioned

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