MX was purchased from Shanghai Macklin Biochemical Co., Ltd. (China). 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), 4-Pyrrolidinopyridine (4-ppy), PEG-NH2 (2000), dithiodibutyric acid, N-hydroxysuccinimide (NHS), folic acid, DSPE-PEG-NH2 (2000), trichloroacetic acid, DL-Dithiothreitol (DTT) and 4-Dimethylaminobenzaldehyde were purchased from Shanghai Aladdin Reagent Co., Ltd. (China). 1-MT-Boc was purchased from SINO High Goal Chemical Technology Co., Ltd. Dialysis bag was purchased from Yobios Science And Technology Co., Ltd. (China).
Roswell Park Memorial Institute-1640 (RPMI-1640), fetal bovine serum (FBS), trypsin solution, penicillin–streptomycin solution and phosphate buffered saline (PBS, pH 7.4) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China). 4% paraformaldehyde solution, 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and HMGB1 ELISA Kit were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Anti-HMGB1 Rabbit mAb and Anti-Calreticulin Rabbit pAb were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). TritonX-100 and Enhanced ATP Assay Kit were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Recombinant mouse interferon gamma (IFN-γ) was bought from BioLegend, Inc. Kyn and Trp were obtained from Sigma Co., Ltd. BALB/c nude mice (male, 5–6 weeks) were purchased from the Beijing Huafukang Biotechnology Co., Ltd. All animal experiments were approved by the Ethical Committee on Animal Experimentation of Yantai University (Yantai, China) (Figure 1).