Protein Expression Analysis in MCF7 Cells

MCF7 and MCF7/Tam cells were washed with ice-cold PBS for 2 times, and then were added with RIPA lysis buffer. Protein in each sample was analyzed by western blotting as previously reported [7 (link)]. The dilution of each antibodies is: anti-S100A8 (1:500) (Proteintech, Chicago, USA), anti-HRD1 (1:1000) (Abcam, Cambridge, UK), anti-GAPDH (1:4000) (Abcam, Cambridge, UK), anti-HA-tag (1:1000) (Proteintech, Chicago, USA), anti-His-tag (1:1000) (Santz Cruz, CA, USA), anti-Myc-tag (1:1000) (Proteintech, Chicago, USA).

Free full text: Click here

Wang Y., Guo A., Liang X., Li M., Shi M., Li Y., Jenkins G., Lin X., Wei X., Jia Z., Feng X., Su D, & Guo W. (2017). HRD1 sensitizes breast cancer cells to Tamoxifen by promoting S100A8 degradation. Oncotarget, 8(14), 23564-23574.

Publication 2017

anti-S100A8 anti-HRD1 anti-GAPDH anti-HA-tag anti-Myc-tag

Antibodies Buffer Cold Dilution Gapdh Mcf7 cells Protein Ripa

Corresponding Organization :

Other organizations : Nanjing Drum Tower Hospital, Nanjing Medical University, Nanjing University of Posts and Telecommunications

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

MCF7 cells
MCF7/Tam cells

dependent variables

Protein expression of S100A8, HRD1, and GAPDH

control variables

Cells were washed with ice-cold PBS for 2 times before lysis
Protein samples were analyzed by western blotting

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!