Quantifying Protein Expression Levels

Protein levels were determined by western blot analysis as previously described.^{37 (link)} Antibodies against β-catenin (#8480), CD44 (#5640), and p-CRKL (#3181) were purchased from Cell Signaling Technology (Danvers, MA), and survivin (AF886) from R&D Systems (Minneapolis, MN). β-actin was used as a loading control. Proteins were visualized with Odyssey infrared imaging system and quantitated using the Odyssey software program (version 3.0; LI-COR Biotechnology, Lincoln, Nebraska).

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Zhou H., Mak P.Y., Mu H., Mak D.H., Zeng Z., Cortes J., Liu Q., Andreeff M, & Carter B.Z. (2017). Combined inhibition of β-catenin and Bcr–Abl synergistically targets tyrosine kinase inhibitor-resistant blast crisis chronic myeloid leukemia blasts and progenitors in vitro and in vivo. Leukemia, 31(10), 2065-2074.

Publication 2017

Odyssey infrared imaging system Odyssey software program

Actin Antibodies Cd44 Crkl Proteins Survivin Western blot analysis β catenin

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, Southern Medical University, Nanfang Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein levels of β-catenin, CD44, p-CRKL, and survivin

control variables

β-actin (used as a loading control)

controls

Positive control: None mentioned
Negative control: None mentioned

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