3D CNFs were obtained from the laboratory of M.D.C. as previously reported (52 , 54 , 55 (link)) (see the Supplementary Materials for details). The bulky 3D scaffolds were cut into thin square slices (lateral size, 3 mm × 4 mm; thickness, 250 to 400 μm) and then secured on standard glass coverslips (Kindler) by PDMS (Sylgard 184 silicone elastomer, Dow Corning) cured at 150°C for 15 min. Thereafter, substrates were cleaned under low-pressure air plasma for 5 min (PDC-32G Plasma Cleaner, Harrick Plasma) and ultraviolet (UV)–sterilized for 20 min before use.
PDMS scaffolds are 3D, self-standing, porous microsponges fabricated as described by Bosi et al. (28 (link)). 2D MWCNT supports were obtained as previously described by Fabbro et al. (8 (link)).
PDMS scaffolds are 3D, self-standing, porous microsponges fabricated as described by Bosi et al. (28 (link)). 2D MWCNT supports were obtained as previously described by Fabbro et al. (8 (link)).
