The MAs and the total extractable lipids were extracted and analyzed as described previously16 (link), except that the (HP)TLC plates for MAME separation were developed in ether/diethyl ether (9:1, v/v, five runs). Purification of MAMEs and compound Z was performed by preparative TLC using silica gel 60 plates (Merck) developed in the above eluent; the compounds were then scraped off and extracted from silica gel three times with diethyl ether.
Lefebvre C., Frigui W., Slama N., Lauzeral-Vizcaino F., Constant P., Lemassu A., Parish T., Eynard N., Daffé M., Brosch R, & Quémard A. (2020). Discovery of a novel dehydratase of the fatty acid synthase type II critical for ketomycolic acid biosynthesis and virulence of Mycobacterium tuberculosis. Scientific Reports, 10, 2112.
Other organizations :
Université de Toulouse, Université Toulouse III - Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Institut Pasteur, Infectious Disease Research Institute
Separation of MAMEs on (HP)TLC plates with ether/diethyl ether (9:1, v/v, five runs)
dependent variables
Total extractable lipids
Purification of MAMEs and compound Z
control variables
Methods for extraction and analysis of MAs and total extractable lipids, as described previously in the referenced publication
controls
No positive or negative controls were explicitly mentioned in the provided information.
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