Labeling and Hybridizing DNA Microarray

DNA was labeled with Dye Cy-5 by a random priming method as previously described (Yang et al., 2017 (link)) and the labeled DNA was purified with QIA quick purification kits (Qiagen, Valencia, CA, United States). SpeedVac (ThermoSavant, Milford, MA, United States) was used to dry DNA at 45°C for 45 min. We hybridized DNA with GeoChip 4.0 on a MAUI hybridization station (BioMicro, Salt Lake City, UT, United States) at 42°C for 16 h, as described previously (Yue et al., 2015 (link)). The slides were scanned using a NimbleGen MS200 scanner (Roche, Madison, WI, United States) at 633 nm using 100% laser power and 75% photomultiplier tube gain. Signal intensities were quantified by scanned images.