Based on the published N. lugens genomic and transcriptomic data (Xue et al., 2014 (link); Wan et al., 2015 (link)), NlHR3 and NlFTZ-F1 homologies were identified and their sequences were confirmed by the reverse transcription polymerase chain reaction (RT-PCR) using primers, as shown in Supplementary Table S1 . The PCR product was gel purified, ligated into the vector TOPO2.1 (Invitrogen, Carlsbad, CA), and transformed into Escherichia coli DH5α competent cells (Novagen, Darmstadt, Germany). A total of 10 recombinant plasmids from several independent subclones were fully sequenced on the Applied Biosystems 3730 automated sequencer (Foster City, CA) from both directions. The newly described transcript variants of NlHR3 and NlFTZ-F1 were submitted to GenBank.
ClustalW2 was used to perform a homologous sequence alignment of HR3 and FTZ-F1 proteins from Nilaparvata lugens, Drosophila melanogaster, Tribolium castaneum, Bombyx mori, Apis mellifera, Aedes aegypti, Pediculus humanus corporis, Blattella germanica, and Acyrthosiphon pisum (Larkin et al., 2007 (link)). The conserved domains were predicted by using the Simple Modular Architectural Research Tool (SMART;http://smart.embl-heidelberg.de/ ) and InterPro: protein sequence analysis and classification (http://www.ebi.ac.uk/interpro/ ).
ClustalW2 was used to perform a homologous sequence alignment of HR3 and FTZ-F1 proteins from Nilaparvata lugens, Drosophila melanogaster, Tribolium castaneum, Bombyx mori, Apis mellifera, Aedes aegypti, Pediculus humanus corporis, Blattella germanica, and Acyrthosiphon pisum (Larkin et al., 2007 (link)). The conserved domains were predicted by using the Simple Modular Architectural Research Tool (SMART;
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